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Journal / Neuroscience / Design, Packaging, og levering af høj Titer…
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Neuroscience

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JoVE Journal Neuroscience
Design, Packaging, og levering af høj Titer CRISPR Retro og Lentivirus via stereotaktisk injektion
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Design, Packaging, og levering af høj Titer CRISPR Retro og Lentivirus via stereotaktisk injektion

Article DOI: 10.3791/53783-v • 11:28 min • May 23rd, 2016
May 23rd, 2016 •
1, 1, 1, 1, 1
1Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth College

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Den CRISPR / Cas9 system tilbyder potentialet til at gøre målrettet genom redigering tilgængelige og overkommelige for det videnskabelige samfund. Denne protokol er beregnet til at vise, hvordan man kan skabe virus, der vil knockout et gen af ​​interesse ved hjælp af CRISPR / Cas9 system og derefter injicere dem stereotaksisk i den voksne mus hjernen.

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Neuroscience lentivirus retrovirus viral pakning høj titer, Intracerebroventrikulær injektion CaPO4 Transfektion
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