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Journal / Immunology and Infection / Preparação de CD4
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Immunology and Infection

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Preparação de CD4

Article DOI: 10.3791/54569-v • 10:00 min • November 8th, 2016
November 8th, 2016 •
1, 1
1Instituto de Investigación en Ciencias Básicas y Aplicadas, Centro de Investigación en Dinámica Celular, Laboratorio de Glicobiología Humana y Diagnóstico Molecular, Universidad Autónoma del Estado de Morelos

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Descreve-se um protocolo de coloração de anticorpo padrão para utilização em microscopia para determinar a expressão e localização da membrana de gangliósidos em repouso e as células naive humanas activadas T CD4 +. Também estão descritos em tempo real utilizando experiências de PCR <40.000 células que não requerem kits adicionais baixos de ARN de entrada.

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Immunology 117 Edição humano de células CD4 + T a activação anticorpo gangliósidos TCR localização microscopia confocal aderência
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