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Immunology and Infection

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JoVE Journal Immunology and Infection
L'induction d'une réponse inflammatoire dans les cultures hépatocytes primaires de souris
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L'induction d'une réponse inflammatoire dans les cultures hépatocytes primaires de souris

Article DOI: 10.3791/55319-v • 08:32 min • March 10th, 2017
March 10th, 2017 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1
1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine, University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Ici, nous montrons une approche enzymatique pour isoler des hépatocytes primaires de souris adultes, et nous décrivons la quantification d'une réponse inflammatoire par ELISA et la PCR en temps réel.

Tags

Immunologie numéro 121 du foie les hépatocytes les cellules cultivées primaires Inflammation interleukine-6 FGF23 CRP LPS maladie rénale chronique
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