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JoVE Journal Bioengineering
Voorspellen van genstyling door middel van de spatiotemporale controle van siRNA-vrijgave van foto-responsieve polymere nanocarriers
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Voorspellen van genstyling door middel van de spatiotemporale controle van siRNA-vrijgave van foto-responsieve polymere nanocarriers

Article DOI: 10.3791/55803-v • 11:53 min • July 21st, 2017
July 21st, 2017 •
1, 1,2, 1
1Department of Chemical and Biomolecular Engineering, University of Delaware, 2Department of Materials Science and Engineering, University of Delaware

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We presenteren een nieuwe methode die foto-responsieve blokcopolymeren gebruikt voor efficiëntere spatiotemporale controle van gen-stilzetting zonder detecteerbare off-target effecten. Bovendien kunnen veranderingen in genuitdrukking voorspeld worden door middel van straightforward siRNA release analyses en eenvoudige kinetische modellering.

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Bioengineering Probleem 125 Polyplex Kinetische Modellering Blokcopolymeer Nucleïnezuurvrijstelling Fluorescentie Correlatie-spectroscopie Celtyping RNAi lichtgevoelig
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