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Journal / Bioengineering / Forutsi gensletting gjennom spatiotemporal…
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JoVE Journal Bioengineering
Forutsi gensletting gjennom spatiotemporal kontroll av siRNA-frigivelse fra fotoresvarende polymere nanocarriers
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Forutsi gensletting gjennom spatiotemporal kontroll av siRNA-frigivelse fra fotoresvarende polymere nanocarriers

Article DOI: 10.3791/55803-v • 11:53 min • July 21st, 2017
July 21st, 2017 •
1, 1,2, 1
1Department of Chemical and Biomolecular Engineering, University of Delaware, 2Department of Materials Science and Engineering, University of Delaware

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Vi presenterer en romanmetode som bruker fotobesvarende blokk-kopolymerer for effektivere spatiotemporal kontroll av genavstenging uten detekterbare off-target-effekter. I tillegg kan forandringer i genuttrykk forutsies ved bruk av enkle siRNA-frigivelsesanalyser og enkel kinetisk modellering.

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Bioengineering Polyplex kinetisk modellering blokk-kopolymer nukleinsyrefrigivelse fluorescenskorrelasjonsspektroskopi celle-mønster RNAi lysfølsom
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