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Entrega de mediada por Virus adeno-asociado de CRISPR para Gene cardiaco en ratones
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Entrega de mediada por Virus adeno-asociado de CRISPR para Gene cardiaco en ratones

Article DOI: 10.3791/57560-v • 09:00 min • August 2nd, 2018
August 2nd, 2018 •
1, 1, 1, 1
1Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program, Ohio State University Wexner Medical Center

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Aquí proporcionamos un protocolo detallado para llevar a cabo en vivo gene cardiaco edición en ratones usando Virus de Adeno-Associated recombinante (rAAV)-mediado entrega de CRISPR. Este protocolo ofrece una estrategia terapéutica prometedora para tratar cardiomiopatía distrófica en distrofia muscular de Duchenne y puede utilizarse para generar nocaut cardiaco específico en los ratones postnatales.

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Medicina número 138 AAV CRISPR Duchenne distrofia muscular distrofina gen edición mdx virus adeno-asociado recombinante
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