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JoVE Journal
Bioengineering

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Dibromomaleimide 二硫化学制备共轭诱导荧光 PEGylated 病毒样粒子
 
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Dibromomaleimide 二硫化学制备共轭诱导荧光 PEGylated 病毒样粒子

Article DOI: 10.3791/57712-v 10:18 min May 27th, 2018
May 27th, 2018

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Summary

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在这里, 我们提出一个程序, 荧光 functionalize 二硫化物 Qβ vip 与 dibromomaleimide。我们描述了 Qβ的表达和纯化, dibromomaleimide 功能分子的合成, dibromomaleimide 和 Qβ的共轭反应。生成的黄色荧光共轭粒子可作为细胞内的荧光探针。

Tags

生物工程 问题 135 纳米材料 病毒样粒子 噬菌体 Qβ (Qbeta) functionalizing 衣壳 荧光 bioconjugation 反应 dibromomaleimide 衍生物 聚乙二醇 (PEG)
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