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Bioengineering

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JoVE Journal Bioengineering
Gjør Bøyning-indusert fluorescerende pegylert Virus-lignende partikler av Dibromomaleimide-disulfide kjemi
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Gjør Bøyning-indusert fluorescerende pegylert Virus-lignende partikler av Dibromomaleimide-disulfide kjemi

Article DOI: 10.3791/57712-v • 10:18 min • May 27th, 2018
May 27th, 2018 •
1, 2, 3, 4
1Department of Chemistry & Biochemistry, University of Texas at Dallas, 2Undergraduate Biology, University of Texas at Dallas, 3Undergraduate Healthcare Studies, University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering, University of Texas at Dallas

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Her presenterer vi en prosedyre for å fluorescently functionalize disulfides på Qβ VLP med dibromomaleimide. Vi beskriver Qβ uttrykk og rensing, syntese av dibromomaleimide-functionalized molekyler og Bøyning reaksjonen mellom dibromomaleimide og Qβ. Den resulterende gule fluorescerende konjugert partikkelen kan brukes som en fluorescens sonde i cellene.

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Bioteknologi problemet 135 nanomaterialer virus-lignende partikler bacteriophage Qβ (Qbeta) functionalizing capsids fluorescens bioconjugation reaksjon dibromomaleimide derivater polyetylenglykol (PEG)
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