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JoVE Journal Neuroscience
Sund hjerne-hypofyse skiver for elektrofysiologiske undersøgelser af hypofyse celler i Teleost fisk
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Sund hjerne-hypofyse skiver for elektrofysiologiske undersøgelser af hypofyse celler i Teleost fisk

Article DOI: 10.3791/57790-v • 07:14 min • August 16th, 2018
August 16th, 2018 •
*1, *1, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences
* These authors contributed equally

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Artiklen beskriver en optimeret protokol for at gøre levedygtige hjerne-hypofyse væv skiver, ved hjælp af teleost fisk medaka (Oryzias latipes), efterfulgt af elektrofysiologiske optagelser af hypofyse celler ved hjælp af patch-clamp-teknik med den perforeret patch configuration.

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Neurovidenskab spørgsmålet 138 hjerne-hypofyse væv-skive Elektrofysiologi fisk patch-clamp endokrine celler
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