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Journal / Biochemistry / HEK 细胞 Mitoribosome 的快速分离
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Biochemistry

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JoVE Journal Biochemistry
HEK 细胞 Mitoribosome 的快速分离
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HEK 细胞 Mitoribosome 的快速分离

Article DOI: 10.3791/57877-v • 09:33 min • October 4th, 2018
October 4th, 2018 •
1, 1, 1, 1
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

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线粒体有专门的核糖体, 与他们的细菌和细胞质的对应物分叉。在这里, 我们展示如何 mitoribosomes 可以获得从他们的本地舱在 HEK 细胞。该方法包括分离悬浮细胞中的线粒体, 并由此纯化 mitoribosomes。

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生物化学 问题 140 线粒体 翻译 核糖体 低温 EM 蛋白质合成 生物化学
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