JoVE Journal
Biochemistry
Biochemistry
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Summary
Please note that all translations are automatically generated.
该协议描述了一种以p53肿瘤抑制蛋白为例从哺乳动物细胞中纯化泛素蛋白的分步方法。泛素化的p53蛋白在严格的非变性和变性条件下从细胞中纯化。