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Journal / Cancer Research / 建立过度表达 dr3 的细胞系,…
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JoVE Journal Cancer Research
建立过度表达 dr3 的细胞系, 评估抗有丝分裂治疗的凋亡反应
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Journal (Cancer Research)
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建立过度表达 dr3 的细胞系, 评估抗有丝分裂治疗的凋亡反应

Article DOI: 10.3791/58705-v • 12:28 min • January 11th, 2019
January 11th, 2019 •
*1, *2, *1, 1,3
1School of Pharmaceutical Sciences, Tsinghua University, 2Department of Radiology, University of California, San Francisco, 3Beijing Advanced Innovation Center for Structural Biology
* These authors contributed equally

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建立一个稳定的细胞系, 过度表达一个有兴趣的基因, 研究基因功能, 可以通过稳定的转化后, 选择单一克隆通过逆转录病毒感染后。在这里, 我们表明, ht29-dr3 细胞系产生这种方式阐明的机制, 死亡受体 3 (dr3) 有助于抗肿瘤诱导的细胞凋亡。

Tags

癌症研究 第143期 稳定细胞系 单菌落 逆转录病毒感染 功能增益 紫杉醇 抗肿瘤 结肠癌 凋亡 死亡受体3
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