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Oprettelse af cellelinjer overekspression DR3 for at vurdere den apoptotiske reaktion på anti-mitotiske Therapeutics
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Chapters
- 00:04Title
- 01:03Generation of DR3 Overexpression Cell Lines
- 05:41Verification of the DR3 Overexpression Cell Line
- 07:46Assessment of the Apoptotic Response of Cells to Antimitotic Agents
- 09:51Results: Assessment of the Apoptotic Response to Anti-mitotic Therapeutics
- 11:15Conclusion
Etablering af en stabil cellelinie overekspression et gen af interesse at studere genfunktion kan gøres ved stabil Transfektion-picking enkelt kloner efter transfecting dem via retroviral infektion. Her viser vi, at HT29-DR3 cellelinjer genereres på denne måde belyse mekanismerne ved hvilken død receptor 3 (DR3) bidrager til antimitotics-induceret apoptose.
