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Journal / Biochemistry / 单粒子冷冻电子显微镜在结构中测定脂质敏感分通道 trpc3…
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Biochemistry

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JoVE Journal Biochemistry
单粒子冷冻电子显微镜在结构中测定脂质敏感分通道 trpc3 的表达与纯化
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单粒子冷冻电子显微镜在结构中测定脂质敏感分通道 trpc3 的表达与纯化

Article DOI: 10.3791/58754-v • 08:27 min • January 7th, 2019
January 7th, 2019 •
*1, *1, 1, 2,3, 1, 1
1Van Andel Institute, 2Vollum Institute, 3Janelia Research Campus
* These authors contributed equally

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该协议描述了用低温电子显微镜测定离子通道结构的技术, 包括用于以最小的努力和毒性在哺乳动物细胞中有效表达基因的杆菌病毒系统, 蛋白质提取、纯化、和质量检查, 样品网格的制备和筛选, 以及数据的收集和处理。

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生物化学 第143期 离子通道 膜蛋白 蛋白质纯化 结构测定 低温电子显微镜 低温 em 杆状病毒
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