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JoVE Journal Neuroscience
Um protocolo de aço inoxidável para isolamento de RNA de alta qualidade do Laser capturar células de Microdissected Purkinje no cerebelo humano Post Mortem
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Um protocolo de aço inoxidável para isolamento de RNA de alta qualidade do Laser capturar células de Microdissected Purkinje no cerebelo humano Post Mortem

Article DOI: 10.3791/58953-v • 09:04 min • January 17th, 2019
January 17th, 2019 •
1, 2,3,4, 1
1Department of Pathology and Cell Biology, Columbia University, 2Division of Movement Disorders, Department of Neurology, Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health, Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, Yale University

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Este protocolo utiliza uma abordagem de mancha-livre para visualizar e isolar as células de Purkinje no tecido fresco congelado do cerebelo humano post mortem através do laser microdissection de captura. O propósito do presente protocolo é gerar quantidades suficientes de RNA de alta qualidade para a sequenciação do ARN.

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Neurociência cerebelo edição 143 células de Purkinje do laser microdissection captura RNA RNAseq tremor essencial
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