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Journal / Developmental Biology / Elektroporation metod för in vivo leverans av…
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JoVE Journal Developmental Biology
Elektroporation metod för in vivo leverans av plasmid DNA i den vuxna Zebrafish telencephalon
Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
Journal (Developmental Biology)
Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
Dissection of Drosophila melanogaster Third Instar Larval Brain for Live-Cell Imaging (Video) | JoVE
Journal (Developmental Biology)
Dissection of Drosophila melanogaster Third Instar Larval Brain for Live-Cell Imaging (Video) | JoVE
 
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Elektroporation metod för in vivo leverans av plasmid DNA i den vuxna Zebrafish telencephalon

Article DOI: 10.3791/60066-v • 06:02 min • September 13th, 2019
September 13th, 2019 •
1,2, 1,3,4
1Institute of Stem Cell Research, Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences, Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center, Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy)

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Presenteras här är en elektroporation metod för plasmid DNA leverans och ependymoglial cell märkning i den vuxna zebrafiskar telencephalon. Detta protokoll är en snabb och effektiv metod för att visualisera och spåra enskilda ependymoglial celler och öppnar upp nya möjligheter att tillämpa elektroporation till ett brett spektrum av genetiska manipulationer.

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Utvecklingsbiologi Electroporation ependymoglia in vivo cell märkning plasmid leverans zebrafiskar telencephalon genredigering
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