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Journal / Genetics / Generation von Chimeric Axolotls mit Mutant…
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Genetics

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Generation von Chimeric Axolotls mit Mutant Haploid Limbs Durch Embryonale Grafting
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Generation von Chimeric Axolotls mit Mutant Haploid Limbs Durch Embryonale Grafting

Article DOI: 10.3791/60156-v • 07:17 min • January 29th, 2020
January 29th, 2020 •
1, 1, 1
1Department of Molecular, Cellular, and Developmental Biology, Yale University

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Dieses Ziel dieses Protokolls ist es, chimäre Axolotle mit haploiden Vorderbeinen aus Cas9-mutagenisiertem Spendergewebe unter Verwendung embryonaler Gewebetransplantationstechniken zu produzieren.

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Genetik Ausgabe 155 Axolotl Haploid Gewebetransplantation Transplantation Regeneration Gliedmaßen Gliedmaßenknospe Chimäre CRISPR Cas9 Multiplex-Mutagenese
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