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Journal / Developmental Biology / Isolatie en differentiatie van primaire…
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JoVE Journal Developmental Biology
Isolatie en differentiatie van primaire Myoblasten van muis skeletspieren Explants
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Isolatie en differentiatie van primaire Myoblasten van muis skeletspieren Explants

Article DOI: 10.3791/60310-v • 06:53 min • October 15th, 2019
October 15th, 2019 •
1, 1
1Scripps Research, Department of Molecular Medicine

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Myoblasten zijn prolifererende precursor cellen die differentiëren om polynucleated myotubes en uiteindelijk skeletspieren myofibers vormen. Hier presenteren we een protocol voor efficiënte isolatie en cultuur van primaire myoblasten van jonge volwassen muis skeletspieren. De methode maakt moleculaire, genetische, en metabole studies van spiercellen in de cultuur.

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Ontwikkelingsbiologie uitgave 152 Myoblasten myotubes muis spier explant weefselcultuur primair stamcellen differentiatie
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