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Journal / Immunology and Infection / Immunofluorescens farvning ved hjælp af IBA1 og…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Immunofluorescens farvning ved hjælp af IBA1 og TMEM119 til Mikroglial tæthed, morfologi og perifer myeloid celle infiltration analyse i muse hjernen
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
Journal (Immunology and Infection)
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
Journal (Immunology and Infection)
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
 
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Immunofluorescens farvning ved hjælp af IBA1 og TMEM119 til Mikroglial tæthed, morfologi og perifer myeloid celle infiltration analyse i muse hjernen

Article DOI: 10.3791/60510-v • 10:40 min • October 27th, 2019
October 27th, 2019 •
1,2, 1,2, 1,3, 1,2,4, 1,2,4, 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

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Denne protokol beskriver en trin-for-trin arbejdsgang for immunfluorescent cofarvning af IBA1 og TMEM119, foruden analyse af mikroglial tæthed, distribution, og morfologi, samt perifer myeloid celleinfiltration i mus hjernevæv.

Tags

Immunologi og infektion mus hjerne microglia myeloide celler neuroimmunologi immunofluorescens IBA1 TMEM119 mikroskopi
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