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Journal / Immunology and Infection / Immunofluorescensfärgning med IBA1 och TMEM119…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Immunofluorescensfärgning med IBA1 och TMEM119 för Mikroglial densitet, morfologi och perifer myeloid cell infiltration analys i mushjärna
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Immunofluorescensfärgning med IBA1 och TMEM119 för Mikroglial densitet, morfologi och perifer myeloid cell infiltration analys i mushjärna

Article DOI: 10.3791/60510-v • 10:40 min • October 27th, 2019
October 27th, 2019 •
1,2, 1,2, 1,3, 1,2,4, 1,2,4, 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

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Detta protokoll beskriver ett steg-för-steg-arbetsflöde för Immunofluorescerande cofärgning av IBA1 och TMEM119, förutom analys av mikroglial densitet, distribution och morfologi, samt perifer myeloisk cell infiltration i mus hjärnvävnad.

Tags

Immunologi och infektion mus hjärna microglia myeloida celler Neuroimmunologi immunofluorescens IBA1 TMEM119 mikroskopi
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