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Journal / Biochemistry / Uso de Termoforese microescala para medir…
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Biochemistry

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JoVE Journal Biochemistry
Uso de Termoforese microescala para medir interações proteína-lipídicas
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Uso de Termoforese microescala para medir interações proteína-lipídicas

Article DOI: 10.3791/60607-v • 04:45 min • February 10th, 2022
February 10th, 2022 •
*1, *2, *1,3,4, 1,3,4, 1,3
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Chemistry, University of South Florida, 3Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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A termoforese de microescala obtém constantes de ligação rapidamente a baixo custo de material. A termoforese de microescala rotulada ou sem rótulo está comercialmente disponível; no entanto, a termoforese livre de rótulos não é capaz da diversidade de medidas de interação que podem ser realizadas usando rótulos fluorescentes. Fornecemos um protocolo para medições de termoforese rotuladas.

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Bioquímica Edição 180 Fosfoinosticida Fosfattidylinositol 3-fosfato domínio FYVE Vacuole Lysosome Endosome
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