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Journal / Biochemistry / Overvågning af protein-RNA-interaktionsdynamik in …
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Biochemistry

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JoVE Journal Biochemistry
Overvågning af protein-RNA-interaktionsdynamik in vivo ved høj tidsmæssig opløsning ved hjælp af χCRAC
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Overvågning af protein-RNA-interaktionsdynamik in vivo ved høj tidsmæssig opløsning ved hjælp af χCRAC

Article DOI: 10.3791/61027-v • 09:15 min • May 9th, 2020
May 9th, 2020 •
1, 1, 2, 3, 1, 1, 4, 4, 3, 1
1Centre for Engineering Biology, University of Edinburgh, 2Institute of Cell Biology, University of Edinburgh, 3Department of Molecular Biology and Genetics, Aarhus University, 4UVO3 Ltd.

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Kinetisk tværbinding og analyse af cDNA er en metode, der muliggør undersøgelse af dynamikken i protein-RNA-interaktioner i levende celler ved høj tidsmæssig opløsning. Her beskrives protokollen detaljeret, herunder vækst af gærceller, UV-tværbinding, høst, proteinrensning og næste generations sekventeringsbiblioteksforberedelsestrin.

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Biokemi udgave 159 CRAC KLIP RNA-proteininteraktion UV-tværbinding teknik stressrespons tidsløst
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