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Journal / Biochemistry / Un saggio basato sull'elettrochemiluminescenza…
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JoVE Journal Biochemistry
Un saggio basato sull'elettrochemiluminescenza per le varianti proteiche MeCP2
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Un saggio basato sull'elettrochemiluminescenza per le varianti proteiche MeCP2

Article DOI: 10.3791/61054-v • 07:44 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 2, 3, 1, 1, 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

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L'elettrochemiluminescenza immunoassay (ECLIA) è un nuovo approccio per il rilevamento quantitativo delle varianti della proteina MeCP2 endogene ed esogenamente applicate su un'ampia gamma di lavoro. Qui, viene descritto il protocollo per il MeCP2-ECLIA in un formato 96-well.

Tags

Biochimica Numero 159 ECLIA Sulfo-TAG MeCP2 Proteina Fusione TAT TAT-MeCP2 Sindrome di Rett terapia sostitutiva delle proteine
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