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Journal / Genetics / Ensayo de inmunoprecipitación de ARN metilado…
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JoVE Journal Genetics
Ensayo de inmunoprecipitación de ARN metilado para estudiar la modificación m5C en Arabidopsis
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Ensayo de inmunoprecipitación de ARN metilado para estudiar la modificación m5C en Arabidopsis

Article DOI: 10.3791/61231-v • 08:50 min • May 14th, 2020
May 14th, 2020 •
*1, *2, 3, 1
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
* These authors contributed equally

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El ensayo de inmunoprecipitación de ARN metilado es un método basado en anticuerpos utilizado para enriquecer fragmentos de ARN metilados. Junto con la secuenciación profunda, conduce a la identificación de transcripciones que llevan la modificación m5C.

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Genética Número 159 Metilación del ARN 5-metilcitosina (m5C) inmunoprecipitación (IP) Arabidopsis epitranscriptomics ARNM secuenciación de ARN
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