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Journal / Genetics / Test di immunoprecipitazione dell'RNA metilato…
JoVE Journal
Genetics

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JoVE Journal Genetics
Test di immunoprecipitazione dell'RNA metilato per studiare m5C Modifica in Arabidopsis
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
Journal (Genetics)
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Journal (Genetics)
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
 
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Test di immunoprecipitazione dell'RNA metilato per studiare m5C Modifica in Arabidopsis

Article DOI: 10.3791/61231-v • 08:50 min • May 14th, 2020
May 14th, 2020 •
*1, *2, 3, 1
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
* These authors contributed equally

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Il saggio di immunoprecipitazione dell'RNA metilato è un metodo a base di anticorpi utilizzato per arricchire i frammenti di RNA metilati. Accoppiato con il sequenziamento profondo, porta all'identificazione di trascrizioni che trasportano la modifica m5C.

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Genetica Numero 159 Metilazione dell'RNA 5-metilcitosina (m5C) immunoprecipitazione (IP) Arabidopsis epitranscriptomica mRNA sequenziamento dell'RNA
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