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Neuroscience

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JoVE Journal Neuroscience
Misurare le interazioni transcellulari attraverso l'aggregazione proteica in un sistema di cellule eterologi
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Misurare le interazioni transcellulari attraverso l'aggregazione proteica in un sistema di cellule eterologi

Article DOI: 10.3791/61237-v • 04:47 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 1
1Department of Pharmacology, University of Colorado Denver School of Medicine

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Qui presentiamo un protocollo ottimizzato per misurare rapidamente e semiquantitativamente le interazioni ligando-recettore in trans in un sistema cellulare eterologo utilizzando la microscopia a fluorescenza.

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Neuroscienze Numero 159 aggregazione ligandi sinaptici HEK293T interazioni tra proteine Neurexin LRRTM Adesione cellulare
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