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Journal / Neuroscience / Mesure des interactions transcellulaires par…
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JoVE Journal Neuroscience
Mesure des interactions transcellulaires par agrégation protéique dans un système cellulaire hétéroologeux
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Mesure des interactions transcellulaires par agrégation protéique dans un système cellulaire hétéroologeux

Article DOI: 10.3791/61237-v • 04:47 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 1
1Department of Pharmacology, University of Colorado Denver School of Medicine

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Ici, nous présentons un protocole optimisé pour mesurer rapidement et semiquantitativement les interactions ligand-récepteur dans trans dans un système cellulaire heterologous utilisant la microscopie de fluorescence.

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Neurosciences Numéro 159 agrégation ligands synaptiques HEK293T interactions trans protéiques Neurexin LRRTM Adhérence cellulaire
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