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Bioengineering

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JoVE Journal Bioengineering
Generation og kvantitativ karakterisering af funktionelle og polariserede galde epitelcyster
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
Journal (Bioengineering)
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
Sandwich-like Microenvironments to Harness Cell/Material Interactions
Journal (Bioengineering)
Sandwich-like Microenvironments to Harness Cell/Material Interactions
 
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Generation og kvantitativ karakterisering af funktionelle og polariserede galde epitelcyster

Article DOI: 10.3791/61404-v • 09:55 min • May 16th, 2020
May 16th, 2020 •
*1, *1, 1, 1, 1,2
1Physiopathogénèse et traitement des maladies du foie, Université Paris-Saclay, Inserm U1193, 2ESPCI Paris, Université PSL
* These authors contributed equally

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Tredimensionale (3D) cellulære systemer er relevante modeller til undersøgelse af organogenese. En hydrogel-baseret metode til galdecyster produktion og deres karakterisering er foreslået. Denne protokol optrævler barriererne i 3D karakterisering, med en enkel og pålidelig metode til at vurdere cyste dannelse effektivitet, størrelser, og at teste deres funktionalitet.

Tags

Bioengineering cholangiocytter selvorganisering lumen celle polaritet cyster organogenese galdegange
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