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Journal / Bioengineering / Metylering Specifik Multiplex Droplet PCR med…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Metylering Specifik Multiplex Droplet PCR med hjälp av Polymer Droplet Generator Device för hematologiska diagnostik
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Metylering Specifik Multiplex Droplet PCR med hjälp av Polymer Droplet Generator Device för hematologiska diagnostik

Article DOI: 10.3791/61421-v • 09:05 min • June 29th, 2020
June 29th, 2020 •
1, 1, 2, 1, 1, 1, 1, 1, 1, 1
1Life Sciences Division, National Research Council of Canada, 2Galenvs Sciences, Inc.

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Epigenetiska markörer används för undertypning av vita blodkroppar (WBC) genom kvantifiering av DNA-metyleringsmönster. Detta protokoll presenterar en multiplex droplet polymeras kedjereaktion (mdPCR) metod med hjälp av en termoplastisk elastomer (TPE)-baserade microfluidic enhet för droplet generation möjliggör exakt och multiplex metylering-specifika mål kvantifiering av WBC differential räknas.

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Denna månad i JoVE metylering multiplexdroppe PCR vit blodkropps subtyping epigenetik droppgenerering termoplastisk elastomer bisulfitbehandlat DNA
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