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Journal / Immunology and Infection / Analyse van human natural killer cell metabolisme
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Immunology and Infection

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Analyse van human natural killer cell metabolisme
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Analyse van human natural killer cell metabolisme

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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In dit artikel beschrijven we een methode om glycolyse en mitochondriale ademhaling te meten in primaire menselijke Natural Killer (NK) cellen geïsoleerd van perifeer bloed, in rust of na IL15-geïnduceerde activering. Het beschreven protocol kan gemakkelijk worden uitgebreid tot primaire menselijke NK-cellen die worden geactiveerd door andere cytokines of oplosbare stimuli.

Tags

Immunologie en infectie Probleem 160 Natural Killer cellen metabolisme extracellulaire flux glycolyse mitochondriën ademhaling elektronentransportketen stroomcytometrie
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