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JoVE Journal Immunology and Infection
Zuivering en uitbreiding van muisinvariante Natural Killer T-cellen voor in vitro en in vivo studies
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Zuivering en uitbreiding van muisinvariante Natural Killer T-cellen voor in vitro en in vivo studies

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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We beschrijven een snel en robuust protocol om invariante natural killer T (iNKT) cellen uit muizen milt te verrijken en in vitro uit te breiden tot geschikte aantallen voor in vitro en in vivo studies.

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Immunologie en infectie iNKT cellen expansie muis Vα14 CD1d anti-CD3/CD28 kralen activering lymfocyten
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