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Biochemistry

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JoVE Journal Biochemistry
Fosfoproteomisk strategi för profilering av osmotisk stresssignalering i Arabidopsis
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Fosfoproteomisk strategi för profilering av osmotisk stresssignalering i Arabidopsis

Article DOI: 10.3791/61489-v • 05:47 min • June 25th, 2020
June 25th, 2020 •
1,3, 2, 3,4, 5
1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

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Presenteras här är en fosfoproteomic strategi, nämligen stoppa och gå extraktionsspetsbaserad fosfoproteomic, som ger hög genomströmning och djup täckning av Arabidopsis fosfoproteome. Detta tillvägagångssätt avgränsar översikten över osmotic stress signalering i Arabidopsis.

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Biokemi Nummer 160 masspektrometri fosfoproteomik proteinfosforylering scentipsmetod Tandem Mass Tag-märkning Arabidopsis,osmotisk stress
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