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Journal / Medicine / 获取细胞毒性和细胞对生物材料的反应…
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获取细胞毒性和细胞对生物材料的反应

Article DOI: 10.3791/61512-v • 09:46 min • July 8th, 2021
July 8th, 2021 •
*1,2,3,4, *2,3,4,5, 1,2,3,4, 2,3,4,5, 2,3,4,6, 2,3,4,6, 2,3,4,7, 2,3,4,5, 1,2,3,4,8, 1,2,3,4
1Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
* These authors contributed equally

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该方法旨在通过使用活力测定和表型分析（包括流式细胞术、RT-PCR、免疫细胞化学以及其他细胞和分子生物学技术）制备可溶性提取物来评估生物材料的细胞毒性。

Tags

撤稿，第 173 期，生物相容性，生物材料，细胞培养，细胞毒性，条件培养基，提取物
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