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Tillgång till cytotoxicitet och cellrespons på biomaterial
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Tillgång till cytotoxicitet och cellrespons på biomaterial

Article DOI: 10.3791/61512-v • 09:46 min • July 8th, 2021
July 8th, 2021 •
*1,2,3,4, *2,3,4,5, 1,2,3,4, 2,3,4,5, 2,3,4,6, 2,3,4,6, 2,3,4,7, 2,3,4,5, 1,2,3,4,8, 1,2,3,4
1Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
* These authors contributed equally

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Denna metod syftar till att utvärdera biomaterialets cytotoxicitet genom beredning av lösliga extrakt, med hjälp av viabilitetsanalyser och fenotypisk analys, inklusive flödescytometri, RT-PCR, immunocytokemi och andra cellulära och molekylärbiologiska tekniker.

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Retraktion utgåva 173 biokompatibilitet biomaterial cellodling cytotoxicitet konditionerat medium extrakt
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