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FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
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FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.

Article DOI: 10.3791/61602-v • 09:26 min • August 25th, 2020
August 25th, 2020 •
1, 2,3, 2,3, 1, 2,3, 1, 1, 2,3, 1, 1,4
1Université de Strasbourg, Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021, 2Université de Strasbourg, UMR 7242, ESBS, 3CNRS, UMR 7242, ESBS, 4Groupe Méthode Recherche Clinique, Hôpitaux Universitaires de Strasbourg

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We describe here a protocol to characterize protein-protein interactions between two highly-differently expressed proteins in live Pseudomonas aeruginosa using FLIM-FRET measurements. The protocol includes bacteria strain constructions, bacteria immobilization, imaging and post-imaging data analysis routines.

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FLIM-FRET Measurements Protein-protein Interactions Live Bacteria Protein Interactions In Native Cellular Environment Fluorescent Protein Expression P.aeruginosa E.coli TOP10 E.coli Helper Bacteria LB Agar Optical Density Centrifuge Resuspend Pellet LB Agar Incubation
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