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Journal / Biology / FLIM-FRET Mätningar av Protein-Protein…
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FLIM-FRET Mätningar av Protein-Protein interaktioner i levande bakterier.
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FLIM-FRET Mätningar av Protein-Protein interaktioner i levande bakterier.

Article DOI: 10.3791/61602-v • 09:26 min • August 25th, 2020
August 25th, 2020 •
1, 2,3, 2,3, 1, 2,3, 1, 1, 2,3, 1, 1,4
1Université de Strasbourg, Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021, 2Université de Strasbourg, UMR 7242, ESBS, 3CNRS, UMR 7242, ESBS, 4Groupe Méthode Recherche Clinique, Hôpitaux Universitaires de Strasbourg

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Vi beskriver här ett protokoll för att karakterisera protein-protein interaktioner mellan två mycket-olika uttryckta proteiner i levande Pseudomonas aeruginosa med hjälp av FLIM-FRET mätningar. Protokollet omfattar bakteriestamkonstruktioner, bakteriemmobilisering, bildbehandling och dataanalysrutiner efter avbildning.

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Biologi FRET-FLIM protein-protein interaktioner Pseudomonas aeruginosa bakterier avbildning fluorescens
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