Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Single-Cell Electroporation på tværs af…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience
Single-Cell Electroporation på tværs af forskellige organotypiske Slice Kultur Af Mouse Hippocampal Excitatory og klasse-specifikke hæmmende neuroner
Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
Journal (Neuroscience)
Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
Generation and On-Demand Initiation of Acute Ictal Activity in Rodent and Human Tissue
Journal (Neuroscience)
Generation and On-Demand Initiation of Acute Ictal Activity in Rodent and Human Tissue
 
Click here for the English version

Single-Cell Electroporation på tværs af forskellige organotypiske Slice Kultur Af Mouse Hippocampal Excitatory og klasse-specifikke hæmmende neuroner

Article DOI: 10.3791/61662-v • 06:21 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1,3, 1
1Brudnick Neuropsychiatric Research Institute, Department of Neurobiology, University of Massachusetts Medical School, 2Interdisciplinary Graduate Program, University of Massachusetts Medical School, 3UMMS MD/PhD Program, University of Massachusetts Medical School
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Præsenteret her er en protokol for enkelt-celle elektroporation, der kan levere gener i både excitatoriske og hæmmende neuroner på tværs af en række in vitro hippocampal skive kultur aldre. Vores tilgang giver præcis og effektiv ekspression af gener i individuelle celler, som kan bruges til at undersøge celle-autonome og intercellulære funktioner.

Tags

Neurovidenskab enkeltcellet elektroporation genlevering neuron interneuron elektrofysiologi hippocampus organotypic skive kultur mus
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter