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Journal / Biochemistry / Enkeltcelleanalyse av transkripsjonsaktive…
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Biochemistry

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JoVE Journal Biochemistry
Enkeltcelleanalyse av transkripsjonsaktive alleler av enkeltmolekyl fisk
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Enkeltcelleanalyse av transkripsjonsaktive alleler av enkeltmolekyl fisk

Article DOI: 10.3791/61680-v • 06:26 min • September 20th, 2020
September 20th, 2020 •
1,2, 1,3, 1,2, 1,2, 1,2,3,4
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology, Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, 4Department of Pharmacology and Chemical Biology, Baylor College of Medicine

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Enkeltmolekyl RNA fluorescens in situ hybridisering (smFISH) er en metode for å nøyaktig kvantifisere nivåer og lokalisering av spesifikke RNAer på enkeltcellenivå. Her rapporterer vi våre validerte laboratorieprotokoller for behandling, avbildning og bildeanalyse av våtbenker for encellet kvantifisering av spesifikke RNAer.

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Biokjemi Utgave 163 Enkeltmolekyl FISK avbildning bildeanalyse gentranskripsjon
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