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Biology
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Produktion af IgG Fusion Proteiner Transiently Udtrykt i Nicotiana benthamiana
 
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Produktion af IgG Fusion Proteiner Transiently Udtrykt i Nicotiana benthamiana

Article DOI: 10.3791/61774-v 13:29 min January 16th, 2021
January 16th, 2021

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Summary

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Vi beskriver her en simpel metode til udtryk, ekstraktion og rensning af rekombinant menneskelige IgG smeltet til GFP i Nicotiana benthamiana. Denne protokol kan udvides til rensning og visualisering af mange proteiner, der udnytter kolonnekromatografi. Desuden er protokollen kan tilpasses til in-person og virtuelle college undervisning laboratorium, der giver projektbaseret udforskning.

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Biologi antistof monoklonalt antistof molekylær pharming agroinfiltration forbigående udtryk Nicotiana benthamiana grøn fluorescerende protein syre-stabil grøn fluorescerende protein antistof fusion IgG-fusion protein G rensning
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