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Journal / Biochemistry / Spotvariations-Fluoreszenz-Korrelationsspektroskop…
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Biochemistry

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JoVE Journal Biochemistry
Spotvariations-Fluoreszenz-Korrelationsspektroskopie zur Analyse der molekularen Diffusion an der Plasmamembran lebender Zellen
Using High Content Imaging to Quantify Target Engagement in Adherent Cells
Journal (Biochemistry)
Using High Content Imaging to Quantify Target Engagement in Adherent Cells
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Journal (Biochemistry)
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
 
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Spotvariations-Fluoreszenz-Korrelationsspektroskopie zur Analyse der molekularen Diffusion an der Plasmamembran lebender Zellen

Article DOI: 10.3791/61823-v • 05:56 min • November 12th, 2020
November 12th, 2020 •
*1, *2, *1, 3, 4, 2, 1, 2
1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ
* These authors contributed equally

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Dieser Artikel zielt darauf ab, ein Protokoll zum Aufbau eines Fluoreszenz-Korrelationsspektroskopie-Mikroskops (svFCS) zur Messung der molekularen Diffusion an der Plasmamembran lebender Zellen vorzustellen.

Tags

Biochemie Ausgabe 165 Spotvariation Fluoreszenzkorrelationsspektroskopie (svFCS) laterale Diffusion Plasmamembrandynamik molekularer Einschluss Lipid-Nanodomänen Zytoskelett-Geflecht Diffusionsgesetz
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