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Journal / Immunology and Infection / 通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Journal (Immunology and Infection)
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
Journal (Immunology and Infection)
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
 
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通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化

Article DOI: 10.3791/62059-v • 04:30 min • February 4th, 2021
February 4th, 2021 •
*1,2, *1, 3,4, 1,3, *2,5, *1,6, *1,6
1Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine, University of Florence, 3Berlin Institute of Health, 4Department of Cardiology, German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine, Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology, Helios Klinikum Erfurt
* These authors contributed equally

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Summary
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在本研究中，我们描述了从钙化主动脉瓣新鲜样品中分离T淋巴细胞的过程，以及使用流式细胞术分析表征适应性白细胞亚群的T细胞克隆的分析步骤。

Tags

免疫学和感染，第168期，主动脉瓣疾病，主动脉瓣狭窄，T细胞提取，TAVI，白细胞外衣，流式细胞术分析，适应性免疫。
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