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Journal / Immunology and Infection / Undersöka aortaklaffförkalkning via isolering och …
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Undersöka aortaklaffförkalkning via isolering och kultur av T-lymfocyter med hjälp av matarceller från bestrålad buffyrock
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Undersöka aortaklaffförkalkning via isolering och kultur av T-lymfocyter med hjälp av matarceller från bestrålad buffyrock

Article DOI: 10.3791/62059-v • 04:30 min • February 4th, 2021
February 4th, 2021 •
*1,2, *1, 3,4, 1,3, *2,5, *1,6, *1,6
1Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine, University of Florence, 3Berlin Institute of Health, 4Department of Cardiology, German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine, Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology, Helios Klinikum Erfurt
* These authors contributed equally

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I denna studie beskriver vi processen för T lymfocyt isolering från färska prover av förkalkade aorta ventiler och analytiska steg av T cell-kloning för karakterisering av adaptiva leukocyte undergrupper med hjälp av flöde cytometry analys.

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Immunologi och infektion nummer 168 Aorta ventilsjukdom aorta stenos T-celler extraktion TAVI buffy coat flöde cytometri analys adaptiv immunitet.
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