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Journal / Neuroscience / In vitro kvantitativ billeddannelse assay for…
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Neuroscience

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JoVE Journal Neuroscience
In vitro kvantitativ billeddannelse assay for phagocytose af døde neuroblastom celler ved iPSC-makrofager
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In vitro kvantitativ billeddannelse assay for phagocytose af døde neuroblastom celler ved iPSC-makrofager

Article DOI: 10.3791/62217-v • 09:48 min • February 14th, 2021
February 14th, 2021 •
1,2,3, 2, 1, 2, 1
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building, University of Oxford, 3UK Dementia Research Institute, Cardiff University

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Neurodegenerative sygdomme er forbundet med dysregulerede mikrogliafunktioner. Denne artikel skitserer en in vitro assay af fagocytose af neuroblastom celler ved iPSC-makrofager. Kvantitative udlæsninger af mikroskopi er beskrevet for både live-celle time-lapse imaging og fast celle højindhold billeddannelse.

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Neurovidenskab fagocytose efferocytose iPSC-makrofag mikroglia neuron SH-SY5Y højt indhold live-celle time-lapse neurodegeneration apoptotisk
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