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Journal / Neuroscience / Essai d’imagerie quantitative in vitro pour la…
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Neuroscience

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JoVE Journal Neuroscience
Essai d’imagerie quantitative in vitro pour la phagocytose des cellules mortes du neuroblastome par iPSC-Macrophages
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Essai d’imagerie quantitative in vitro pour la phagocytose des cellules mortes du neuroblastome par iPSC-Macrophages

Article DOI: 10.3791/62217-v • 09:48 min • February 14th, 2021
February 14th, 2021 •
1,2,3, 2, 1, 2, 1
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building, University of Oxford, 3UK Dementia Research Institute, Cardiff University

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Les maladies neurodégénératives sont associées à des fonctions microgliales dérégulées. Cet article décrit un test in vitro de phagocytose des cellules de neuroblastome par les macrophages iPSC. Les lectures de microscopie quantitative sont décrites à la fois pour l’imagerie time-lapse sur cellules vivantes et l’imagerie à haut contenu à cellules fixes.

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Neurosciences Numéro 168 phagocytose efférocytose iPSC-macrophage microglie neurone SH-SY5Y haute teneur cellule vivante time-lapse neurodégénérescence apoptotique
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