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Isolatie en kweek van residente cardiale macrofagen van de Murine Sinoatriale en Atrioventriculaire Knoop
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Isolatie en kweek van residente cardiale macrofagen van de Murine Sinoatriale en Atrioventriculaire Knoop

Article DOI: 10.3791/62236-v • 08:43 min • May 7th, 2021
May 7th, 2021 •
1,2,3, 1, 1,3, 1, 1,2,3, 1,2,3, 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

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Het hier gepresenteerde protocol biedt een stapsgewijze aanpak voor de isolatie van cardiale residente macrofagen uit het sinoatriale knooppunt (SAN) en atrioventriculaire knoop (AVN) gebied van muizenharten.

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Geneeskunde Nummer 171 hartgeleidingssysteem sinoatriale knoop atrioventriculaire knoop residente cardiale macrofagen flowcytometrie magnetische geactiveerde celsortering MACS fluorescentie-geactiveerde celsortering microdissectie primaire celkweek
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