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JoVE Journal Biology
Isolering av primær rotte hepatocytter med multiparameter perfusjonskontroll
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Isolering av primær rotte hepatocytter med multiparameter perfusjonskontroll

Article DOI: 10.3791/62289-v • 07:35 min • April 5th, 2021
April 5th, 2021 •
*1, *2, 3, 4, 5, 1, 3, 1,3,4,5,6
1Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, 2College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute, National University of Singapore, 4Institute of Bioengineering and Nanotechnology, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP, Singapore-MIT Alliance for Research and Technology
* These authors contributed equally

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Denne protokollen beskriver bruken av et spesielt intravenøst kateter, standardisert steril engangsrør, temperaturkontroll supplert med sanntidsovervåking og et alarmsystem for to-trinns kollagennaseperfusjonsprosedyre for å forbedre konsistensen i levedyktigheten, utbyttet og funksjonaliteten til isolerte primærrottehedopatocytter.

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Biologi utgave 170 celleisolasjon hepatocytt to-trinns perfusjon kannasjon integrert enhet
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