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To-trinns omvendt transkripsjon dråpe digitale PCR-protokoller for SARS-CoV-2 deteksjon og kvantifisering
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To-trinns omvendt transkripsjon dråpe digitale PCR-protokoller for SARS-CoV-2 deteksjon og kvantifisering

Article DOI: 10.3791/62295-v • 07:54 min • March 31st, 2021
March 31st, 2021 •
1,2,3, 1, 2,3,4, 1, 5, 1,2,3, 1,2,3, 1,2,3, 1, 1, 1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

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Dette arbeidet oppsummerer trinn for å utvikle forskjellige analyser for SARS-CoV-2-deteksjon ved hjelp av et tofarget ddPCR-system. Trinnene er forseggjorte og notater er inkludert om hvordan du kan forbedre analysene og eksperimentytelsen. Disse analysene kan brukes til flere SARS-CoV-2 RT-ddPCR-applikasjoner.

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Denne måneden i JoVE utgave 169 simplex dupleks triplex probe mix quadruplex analyser SARS-CoV-2 RT-ddPCR deteksjon multiplex
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