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JoVE Journal Biology
Protocolos digitais de PCR digital de gotícula de transcrição reversa em duas etapas para detecção e quantificação de SARS-CoV-2
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Protocolos digitais de PCR digital de gotícula de transcrição reversa em duas etapas para detecção e quantificação de SARS-CoV-2

Article DOI: 10.3791/62295-v • 07:54 min • March 31st, 2021
March 31st, 2021 •
1,2,3, 1, 2,3,4, 1, 5, 1,2,3, 1,2,3, 1,2,3, 1, 1, 1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

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Este trabalho resume as etapas no desenvolvimento de ensaios diferentes para a detecção de SARS-CoV-2 usando um sistema ddPCR de duas cores. As etapas são elaboradas e notas foram incluídas sobre como melhorar os ensaios e o desempenho do experimento. Estes ensaios podem ser usados para aplicações SARS-CoV-2 RT-ddPCR.

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Este mês em JoVE Edição 169 simplex duplex triplex probe mix quadruplex ensaios SARS-CoV-2 RT-ddPCR detecção multiplex
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