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Método de expansión celular Natural Killer (NK) y CAR-NK utilizando línea de células B modificada con IL-21 unida a la membrana
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Método de expansión celular Natural Killer (NK) y CAR-NK utilizando línea de células B modificada con IL-21 unida a la membrana

Article DOI: 10.3791/62336-v • 11:02 min • February 8th, 2022
February 8th, 2022 •
1,2, 1, 1, 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

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Aquí, presentamos un método para expandir el asesino natural de sangre periférica (PBNK), las células NK de los tejidos hepáticos y las células NK del receptor de antígeno quimérico (CAR) derivadas de células mononucleares de sangre periférica (PBMC) o sangre del cordón umbilical (CB). Este protocolo demuestra la expansión de las células NK y CAR-NK utilizando células alimentadoras 221-mIL-21, además de la pureza optimizada de las células NK expandidas.

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Investigación del cáncer Número 180
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