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Journal / Cancer Research / Natural Killer (NK) og CAR-NK…
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Cancer Research

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JoVE Journal Cancer Research
Natural Killer (NK) og CAR-NK celleudvidelsesmetode ved hjælp af membranbundet-IL-21-modificeret B-cellelinje
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Natural Killer (NK) og CAR-NK celleudvidelsesmetode ved hjælp af membranbundet-IL-21-modificeret B-cellelinje

Article DOI: 10.3791/62336-v • 11:02 min • February 8th, 2022
February 8th, 2022 •
1,2, 1, 1, 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

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Her præsenterer vi en metode til at udvide perifert blod naturlig dræber (PBNK), NK-celler fra levervæv og kimære antigenreceptor (CAR) -NK-celler afledt af perifere blodmononukleære celler (PBMC'er) eller navlestrengsblod (CB). Denne protokol demonstrerer udvidelsen af NK- og CAR-NK-celler ved hjælp af 221-mIL-21 feederceller ud over den optimerede renhed af ekspanderede NK-celler.

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